Share a copy of RaFD Dataset?

I tried to get this dataset from the original link but no one approved my requests for weeks. Can someone share with me a private link to use? You can send it to my institutional email:...

14 February 2021 1,908 1 View

Help with Amyloid beta detection protocol (western-blotting)?

Hi everyone, I am a first year PhD student working on amyloidosis. I would like some tips and tricks to crack efficiently my current nightmare, i.e. the western blotting of the Amyloid beta and...

11 February 2021 2,513 3 View

Does viral RNA extraction from nasopharyngeal swabs require pre-centrifugation with supernatant collection?

06 February 2021 323 3 View

Aspen plus (hydrometallurgy set-up) & reactions solid-liquid ?

I have to simulate a hydrometallurgical process where there are the following phases: - leaching (solid-liquid reactions) - cementation (solid-liquid reactions) - electrowinning (energy ) -...

30 January 2021 9,225 3 View

How to prediction non-visible parts of microscopic pictures?

Dear all, I like to ask if someone has experience with evaluating pictures towards the prediction of non-visible parts. I have confocal-laser-scanning microscopic pictures of yeast cells and want...

28 December 2020 4,309 11 View

Coronal or Sagittal mouse brain sections to visualise vessels?

Hello, I am a first year PhD student and I am working on Cerebral amyloid angiopathy (CAA). I would like to investigate the presence of my proteins of interest in a CAA/Alzheimer's disease mouse...

05 December 2020 907 3 View

What is the cleanest / best way to handle different software dependencies / configurations?

Dear RG users, I noticed that several toolboxes developed for python are compatible with some specific releases and not with others and in general there is a precise set of tools defining the...

30 November 2020 4,545 5 View

Why maximum allowable limits for toxic metals or heavy metals in agricultural soils vary among countries and institutions?

For example, the maximum allowable limit for USEPA in agricultural soils are very low when compared to Tanzania's maximum allowable limits see figure below. Does this can affect the management...

23 November 2020 5,052 10 View

What is the best way to get organoids of the same size?

I would like to get organoids of the same size. We filter organoids using a 100 um filter and then pass that filtrate through a 40 um filter followed by flipping the 40 um filter. Then we wash the...

22 November 2020 9,391 3 View

How to match Matplotlib color mapping in a R ggscatter plot (or ggplot)?

I created this shinyapp and am having a hard time seeing the image I thought I was...

13 October 2020 223 2 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Chlorinated organic formation in odor control scrubbers using hypochlorite?

I'm involved in a study of odor control technologies for municipal wastewater treatment plant. One of the control options involves a chemical 2-stage (acid/alkaline) packed bed scrubber. The...

03 March 2021 3,661 2 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

Dapi fluorescence protocol for colon cell lines ?

28 February 2021 2,534 2 View

Does RNAiMAX transfection reagent work in serum free medium?

I diluted siRNA and RNAiMAX in opti-MEM and added to the cells which they were in the growth medium. Is it a right way? or should I culture cells in the opti-MEM medium for a while and not in...

26 February 2021 10,041 3 View

Has anyone had luck using cell lines other than HEK 293 (such as COS1) to package lentivirus?

I am looking to package lentivirus in endothelial cells but I am opposed to using 293 cells due to personal ethics regarding the source. I have done some reading and saw that COS1, COS7, & CHO...

25 February 2021 3,030 1 View

Unknown debris sheets observed in cell culture flasks?

Hi, We culture neural progenitor cells in T175 culture flasks coated with PLO-Laminin. We have successfully grown these cells with our protocol for months. Recently, we observed large sheets...

25 February 2021 3,443 4 View

How do I transfect a large plasmid into PC12 cells with nerve growth factor (NGF) receptor?

I have a large 16 kb plasmid, which I need to transfect into PC12 cells. Lipofectamin 2000 didn't work and with GFP alone the transfection rate is very low. I also tried the neon invitrogen...

25 February 2021 6,635 5 View

How to increase solubility of Poly acrylic acid in Dioxane solvent?

I am working with Polyacrylic acid (100.000Da) and instead of water, I need to use another solvent (dioxane) to dissolve PAA. However, because of the high molecular weight, it's quite hard to...

25 February 2021 2,225 5 View