I used 1X MOPS with 0.67% formaldehyde as both running buffer and 1% agarose gel solution. I denatured my sample and RNA ladder with 22% formaldehyde, 20% formamide, loading dye, and sample in 70C for two minutes. I ran my gel on a horizontal Owl Separation System mini-gel B1 at 100V for 50min. All proportions and volumes used were based on or modified from Brody 2004. I assume that if I had degraded RNA, or miscalculated my sample at least the marker would show up. But I got nothing at all. The XCFF and BPB ran as expected. Using the same sample I got excellent results from Bio-Rad's Experion StdSens RNA electrophoresis.
Other than somehow having acute temporary amnesia where I didn't load any DNA at all, how can this happen?
*yes the gel is oversaturated with EtBR. I couldn't believe I would have nothing and let it sit again, longer in stain solution. Didn't help though.
http://www.biotechniques.com/BiotechniquesJournal/2004/October/Ultra-fast-high-resolution-agarose-electrophoresis-of-DNA-and-RNA-using-low-molarity-conductive-media/biotechniques-117338.html