I used 1X MOPS with 0.67% formaldehyde as both running buffer and 1% agarose gel solution.  I denatured my sample and RNA ladder with 22% formaldehyde, 20% formamide, loading dye, and sample in 70C for two minutes. I ran my gel on a horizontal Owl Separation System mini-gel B1 at 100V for 50min.  All proportions and volumes used were based on or modified from Brody 2004. I assume that if I had degraded RNA, or miscalculated my sample at least the marker would show up. But I got nothing at all.  The XCFF and BPB ran as expected.  Using the same sample I got excellent results from Bio-Rad's Experion StdSens RNA electrophoresis. 

Other than somehow having acute temporary amnesia where I didn't load any DNA at all, how can this happen? 

*yes the gel is oversaturated with EtBR. I couldn't believe I would have nothing and let it sit again, longer in stain solution.  Didn't help though. 

http://www.biotechniques.com/BiotechniquesJournal/2004/October/Ultra-fast-high-resolution-agarose-electrophoresis-of-DNA-and-RNA-using-low-molarity-conductive-media/biotechniques-117338.html

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