Hi all,
Do you guys have any idea to use the tamapin/apamin in behavior experiments? I want to use this drug to block the SK2 channel and see the effect in learning behavior. I am not sure which concentration and how should I administrate this drug in the primary motor cortex of mouse. Does any one have done before?
Thanks
Shahid
I am not sure but you might want to check out the below article and see if it has been cited in anywhere else;
J Biol Chem. 2002 Nov 29;277(48):46101-9. Epub 2002 Sep 17.
Tamapin, a venom peptide from the Indian red scorpion (Mesobuthus tamulus) that targets small conductance Ca2+-activated K+ channels and afterhyperpolarization currents in central neurons.
Pedarzani P1, D'hoedt D, Doorty KB, Wadsworth JD, Joseph JS, Jeyaseelan K, Kini RM, Gadre SV, Sapatnekar SM, Stocker M, Strong PN.
Cheers
Nick
Apamin is highly potent. The following paper used 10 micromolar/ 0.5 microliters in the infralimbic prefrontal cortex of rats. Good luck.
Article Modulating fear extinction memory by manipulating SK potassi...
Thank you guys for your comments. I got some good publication though these links. Hope these will be very helpful for me.
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