I am performing bacterial viability assay, using resazurin for my analysis. I have tried using from the range of 0.015% to 2%. Have been able to achieve results, but I am unsure at what level resazurin might get toxic to the bacterial cells and give me false positive results. Can anybody provide an answer with a reference?
Dear Tejaswini Kalkundri, resazurin used by 10% depending on the final volume of your culture.
Ideally 10% resazurin recommended by manufacturer, it also work equally fine at 8%.
Best
You can go as low as 5% resazurin.
You can get more information on the following manuscript:
https://aac.asm.org/content/49/7/2612
Also, I recommend you to check by using different amounts of resazurin 0-10% (or even higher) if you are working in the linear range of your apparatus. A careful calibration is essential for this assay.
- Badges
- Science topic
- Similar topics
- Psychology