What concentration of protein(mg/ml) is appropriate for gel filtration(Sephadex G-75) and ion exchange (DEAE-cellulose) chromatography? (Gel filtration column (120*1.5cm) and ion exchange column (25*2cm).)
José Marcos Gontijo Mandarino
This concentration depends on many different conditions of the chromatography but you can use a concentration between 0.5 to 1.0 mg/mL
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Yong-Ming Wei
Depend on the column capacity, the flow rate you run and the time for one cycle of operation. C0*Q*t should be about the capacity of column.
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