would you use MDC for autophagy detecting? If so, you can incubate cells with 0.05mM MDC for 10 min.

i did it, even on 0.025mM concentration cells start leaving the surface of the cover slip which was coated with poly L-lysine

Try to cover something else, a charged similar compound, like mdc disturbs the cells' attachement.

can you suggest me the compound which can be used in place of poly L-lysine

neuronal cells, might be lamin is the best but expensive, or try gelatin or collagen. you can accept some loss of cells on PLO or PLL if you still get meaningful staining. good luck.

http://biosciencetools.com/catalog/links/GlassCoating.htm