Ibrar Siddique

6 Questions 7 Answers 0 Followers

Questions related from Ibrar Siddique

How can I avoid the granules like structure on the surface of the cells?

Hello Everyone,We are facing a problem in all cell lines running in our culture room. After two or three passages, the cells start showing the granules like structure on its surface. They...

04 April 2016 1,542 4 View

Regarding the MTT assay; shall I process my results or perform a cell viability assay?

I did an MTT assay on HT-22 cell line of my drug. I got the higher reading in my treatment sample, it shows that the drug is proliferating the cells, because the reading becomes higher, when I...

11 November 2015 4,142 3 View

My fusion protein has a MW of about 46KD. The band appears at about 40 KD, in purified form and in the lysate too. Can any one tell me the reason?

I have cloned a gene in pgex-4t1 vector, which is gst tag. My protein is about 20KD. While band appear at about 40KD. I have confirmed the clone by sequencing and double digestion. And also...

11 November 2014 1,000 7 View

How can I measure cholesterol level in my cell lysate?

I want to see the change in the cholesterol level in my cells after the treatment. I am using the SH-SY5Y cell line.

11 November 2014 4,623 12 View

What concentration of monodensylchadaverine should be used in neuroblastoma cell line(SH-SY5Y)?

I have tried conc of the dye from o.o25mM to the 0.1mM but the cell death starts on all conc

12 December 2013 7,562 5 View

No puncta appears after the transfection of eGFP-LC3, why?

I am working on autophagy, I have seen the downrwgulaion and upregulation of autophagy by LC3B using Western Blot. When I tried to show this using eGFP-LC3 transfection, whole cell fluoresce, and...

01 January 1970 5,646 5 View