I have been running some ChIP-seq experiments in P. aeruginosa bacterial cultures. Following chromosome immunoprecipitation and decrosslinking I measured my DNA concentration on a Qubit fluorometer and had DNA centrations of between 0.1 and 0.3 ng/uL. I was wondering if anyone would be able to provide some insight into the DNA concentrations that are typically received following the decrosslinking step of the ChIP prior to beginning library preparation.
Hi Christopher Kesthely ,
I have performed Chip, not on Bacterial cultures but on mammalian cancer cells. I typically had used around 1500-2000K cells per Chip and at the end of Chip, I got around 50-100ng/uL (Qubit).
Hope this helps.
Good Luck!
Thank you for your answers. I have been using roughly 25-50ng of sheared input DNA. From RNA-seq experiments, I know that the ligand I am studying regulates roughly 400 genes, so I estimate I am looking for roughly 100 bacterial promoter elements (maybe a few more or less depending on secondary and tertiary expression in the RNA-seq data).
- Badges
- Science topic
- Similar topics
- Biological Science
- Zoology
- Ornithology