Hello, I am trying to get raman scattering signal from my spectrometer. Previously I had a setup for fluorescence spectrometer and it was working pretty good with good signal intensity. Now, I am trying to get raman scattering from the same setup. My general setup is:
477nm excitation laser
250 s^-1 chopper
sample cell
477 nm filter placed at right angle to the sample cell
monochrometer and the detection system (at right angle).
I was hoping to get a raman scattering signal from benzene. But instead I ended up getting a signal at 606 nm. I made sure that the benzene I used was not contaminated by running it in a raman spectrometer and the signal peak was at 1000 cm^-1. I wanted to know if the 606nm signal that I got for benzene in my setup corresponds to something. Or may be its just a calibration error? Also, I wanted to ask if I have the wrong system setup and need to make some changes to make the fluorescence to raman scattering conversion. Also, I would be grateful if I could get help on figuring out and calculating proper raman scattering signals.
Thanks a lot in advance.
Just one small point, from my personal experience.
The one thing that is very important here is to understand that the Raman signal is several orders of magnitude weaker than the fluorescence. So there's a good chance you will not get any signal, and definitely not a good signal with the fluorescence setup.
My supervisor used to tell me that measuring Raman in a fluorescence device is just a good way to get confused, and you would always have to explain yourself later. I would advice against it.
Xu Feng Amir Nakar Thank you for the reply. I switch the excitation beam to 532nm. I figured the 606 nm signal was simply some contaminant in the sample itself. I have added further optics in order to better collect the scattering signals.
Again, Thank you for the suggestions.
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