Dear Rahele Zareshahi ,

• As Ahmed said, Ultrasonic Assisted Extraction (UAE) is a great methods to extracrt bioactive compounds. You could try my methods for polyphenols which have recently published:

  Article Evaluation of the chemical composition and nutritional poten...

• Moreover, you could also try Ultrasonic Assisted Enzyme Extraction (UAEE) for that. My related paper will be relased soon.
• You could also see some methods or solvent which may related to your substrate. Here is my recent published review paper for extraction methods for polyphenol:

  Article Phlorotannins: A review of extraction methods, structural ch...

Hope this information could help you.

Best regards,

Weihao

Dear Rahele,

The extraction procedure involves soaking the crude drug, in the form of either a powder or a decoction (kasaya), for a specified period of time, during which it undergoes fermentation and generates alcohol in situ; this facilitates the extraction of the active constituents contained in the plant material.

Decoction is a method of extraction by boiling herbal or plant material (which may include stems, roots, bark and rhizomes) to dissolve the chemicals of the material. It is the most common preparation method in various herbal-medicine systems.

Ethanol is widely used in oil extraction for its ability to extract a potent amount of oil. Ethanol is very effective in separating oils from the plant material, resulting in large extraction quantities.Methanol was identified as the most effective solvent for the extraction, resulting in the highest extraction yield (33.2%) as well as the highest content of phenolic (13.36 mg GAE/g DW), flavonoid (1.92 mg QE/g DW), alkaloid (1.40 mg AE/g DW), and terpenoids (1.25%, w/w).

As a result of modern extraction, isolation techniques and pharmacological testing procedures, new plant drugs usually find their way into medicine as purified substances rather than in the form of galenical preparations. The precise mode of extraction naturally depends on the texture and water content of the plant material being extracted. There are two types of procedure for obtaining organic constituents-

a. Cold extraction

b. Hot extraction.

The extract obtained is then concentrated and constituents are separated by different methods such as chromatography. As a standard precaution against loss of material, concentrated extracts should be stored in the refrigerator.

Extraction at a glance:

Plant collection

Drying

Milling

Powder of the dried part

Macerated with 96% ethanol for 14 days

Filtration by cotton plug

Filtration by whatman filter paper

Evaporation

Crude dried ethanol extract

If you are interested have a look on the mentioned article :

Article Phytochemical investigation and assessment of pharmacologica...

Thank you.

Ultrasonic Assisted Extraction (UAE) is the method as far as I know.

Pulverize and select solvents that are suitable in extracting specific components.

https://www.nature.com/articles/s41598-020-78847-5

https://dst.gov.in/new-technology-can-enhance-solubility-poorly-water-soluble-multi-herbal-extracts-using

https://www.coleparmer.com/tech-article/solvent-extraction-method-ethanol

https://doi.org/10.3390/molecules25204672

https://doi.org/10.4103/0250-474X.70492

https://www.researchgate.net/post/Which_is_the_best_solvent_for_herbal_extraction,kindly check this link.