Hello!
I will be extracting RNA from whole juvenile Zebrafish samples but I do not have any liquid nitrogen available. Does anyone know any alternative recommendations that we can use to get a good yield?
Will also be using Promega ReliaPrep RNA Tissue Miniprep System kit as this is the one that is available in the lab.
Thank you!
I did it for zebrafish larvae. The larvae were anesthetized, washed, and put in an Eppendorf tube with glass beads. The total RNA was isolated with a TRIzol reagent. The larvae were homogenized in the presence of TRIzol and glass beads, the chloroform was added and incubated for 3 min. After incubation, the mixture was centrifuged at 12,000 g for 15 min at 4’C. Three layered solutions formed, the upper clear portion containing RNA was pipetted to a new Eppendorf tube and ice-cold isopropanol was added and the tube was inverted several times to disperse any visible precipitate.
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