I would like to know if there are interferences when performing Monospot Test
This is called a false-positive result, and it may occur in people with:
- Hepatitis.
- Leukemia or lymphoma.
- Rubella.
- Systemic lupus erythematosus.
- Toxoplasmosis. Antibodies are produced by your immune system to fight infections. False results include: Monospot tests can be false-negative about 10% to 15% of the time, particularly in the early stages of the illness
Monospot test is a common blood test which is used to rapidly detect infectious mononucleosis caused by the Epstein-Barr virus. According to Stuempfig, this test consists of multiple interfering factors, which considerably reduce its sensitivity and therefore its specificity. Regarding the sensitivity of the test, there are various factors that contribute to false negative test results such as patient age and patient tested before 1-2 weeks of symptom onset. On the other hand, certain case reports have described false positives for conditions like Cytomegalovirus, HIV, lupus, lymphoma, rubella, and herpes simplex virus.
Stuempfig, N., & Seroy, J. (2019, April 11). Europe PMC. European PMC. https://europepmc.org/article/NBK/nbk539739
False positive due to cross reacting antibodies
and False negative due to sensitivity of the test and timing of the test
