What can be the cause of darkening of whole blood culture?

16 October 2023 0 9K Report

We culture whole blood in RPMI media (10x dilution) for 48 hours without stimulation for RILA assay on 37C. Sometimes the blood darkens, but it is random, usually only one from the parallel samples. The sample next to the darkened one is ok, but another one on the other side of the thermostat also darkened. Some of these darkened samples seem to contain more debris measured by flow cytometry, but not all. Does anybody have any suggestions?

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