Lately I have been researching biological samples with open source image processing software. I want to make the following measurements:
Cell count;
Cell tracking over time;
Doubling time;
Volumetric data.
I have been able to make accurate cell counts with Cell Profiler and Fiji (ImageJ). (Automated) tracking over time was also successful with Fiji (TrackMate and MTrackJ plugins). Now only the doubling time and volumetric data calculation remain unsolved. The Fiji plugins for volume calculations (VolumeJ etc.) all require multiple stacks of images but there must also be a way to estimate the volume based on single stack images (with multiple time frames), right?
As for the doubling time, I reckon this would be not so hard to make as a plugin when the time interval between each frame and the cell count in each frame is known.
So basically there are multiple questions here: First I'd like to know why you use a certain program over others and second if you have any tips or suggestions for volume and/or doubling time calculations.
Thanks in advance!
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