What are the validated exon-exon junction primers for lncRNA MEG3 variant 1 that produce sharp RT-PCR or qPCR bands?

Seyede Samane Nabavian @Seyede-Samane-Nabavian

05 August 2025 1 8K Report

Dear professors,

Could you kindly recommend a primer sequence for lncRNA MEG3 variant 1 that is designed across the exon-exon junction, has been previously validated in published studies, and has produced sharp and distinct bands in RT-PCR or qPCR expression analysis?

Katie A S Burnette

Have you tried a literature search? The odds that a specialist in this exact question giving you a timely answer are pretty low. This is the second posting where you are hoping to find a quick answer to a very specific question. And there might NOT even be a published answer.

Seriously, your advisor is going to expect you to have a bit more grit.

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