Does the universal extractant absolutely replace the traditional extractants used in various soil analyses?

Kindly share your experiences or relevant published literature.

04 May 2018 9,411 0 View

Is it possible to determine phosphates in an alkaline medium?

Conventional method of phosphate determination relies on blue/yellow color development in an acidic medium. Does someone have a P determination method in alkaline medium?Please advise!

08 September 2016 7,801 0 View

Best method of extracting fresh leaf sap for nutrient estimation?

Please advise!

07 August 2016 8,376 1 View

Can someone suggest good books on complete physiology + agronomy of potato, groundnut, maize and soybean?

Please suggest!

07 August 2016 8,092 1 View

Why do you think neem oil-coated urea (NOCU) should be miraculously effective?

06 July 2016 8,616 10 View

Are Indian soils absolutely deficient in nitrogen?

I urge all colleagues to kindly put their experiences and opinions.

06 July 2016 8,437 15 View

Is an average Indian farmer overdosing soils with N and P fertilizers?

Your opinions please!

06 July 2016 4,201 14 View

Does someone think that quoting too much of reference in a discussion group makes the science topic more of "physical"?

It is my personal feeling and observation (may be wrong also) that RG members, when in a scientific discussion, paste a lot of reference materials or paste material from some...

06 July 2016 5,038 3 View

Any prestigious international journal where I should submit my paper on biochar? Any good suggestion?

I want your suggestions!

06 July 2016 3,589 2 View

Is there limited availability of Hyperion EO-1 data for India?

Hi all, I tried getting Hyperion EO-1 data from Earth Explorer but could not find data for my region of interest.Can someone help in this? Thanks MKD

06 July 2016 3,158 0 View

How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Does any one know how i can prepare liver tissue with transmission electron microscope with good fixation?

i have problem in preparation of liver tissue with bad cell organelles and how i can adjust osmolarity

03 March 2021 8,890 1 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

Any specific pipeline or parameter to analyse total transcriptomics study of brain tissue samples?

I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...

27 February 2021 356 6 View

B-mercaptoethanol (BME) protocol?

Hi everybody, I have been running a multiplex IHC which allow me to stain the same tissue with different markers (Primary Ab + Secondary conjugated Ab) (AF487, AF555, AF647). Since I would like to...

27 February 2021 5,028 2 View

Looking for genomic DNA extraction kits from mouse tissue (centrifuge-based, not spin-column)?

Hello. I am looking to extract genomic DNA from mouse brain and lungs and I need a kit that is not spin-column based because I need to scale up the solution amounts for 400mg tissue (most cap at...

25 February 2021 155 2 View

Has anyone tried to scan fluorescence IHC slides with microarray scanner?

I have an old slide microarray scanner (VIDAR Revolution 4200) and would like to give it a "second life", as IHC scanner array (fluorescence dyes), FISH over tissue or similar. Has anyone tried...

24 February 2021 9,210 1 View

Recommendations on homogenizers for RNA isolation?

I will be performing RNA isolation with frozen lung biopsy samples and am in the market for a tissue homogenizer. I am wanting a homogenizer that will fit in a 1.5ml tube. I was looking at the...

24 February 2021 3,623 3 View

No title

22 February 2021 9,932 11 View

How to locate and collect the pineal gland in the amphibian brain?

Our team has been trying to locate and collect the pineal gland in the American bullfrog's brain (Lithobates catesbeianus) for some time now. However, we don't have a standardized protocol for...

18 February 2021 3,453 4 View