DNA aptamers can bind their targets specifically. Eg. the ATP aptamer binds ATP. What are the thermodynamic parameters (deltaG, deltaH, deltaS) for this process? And what are the respective thermodynamic parameters for interaction of a non-target (e.g. GTP) with the ATP aptamer? Any example values?
Dear Kan,
I only am aware of one case: comparison of the binding of cocaine and quinine to a series of DNA aptamers. In the Biochemistry paper that I join to this answer (Biochemistry 2013 52(48): 8652), the Table 1 lists these thermodynamic parameters for the binding of both small organic molecuel to several DNA aptamers.
Hope it answers to your needs.
Best wishes
Pr Philippe Urban
Dear Kan,
Another example, this time concerning the binding of various nitrogen bases to a purine ribosiwtch (not a real DNA apatmer!!). Look at Table 2!
Best regards,
Philippe
Dear Kan,
RNA and DNA aptamers against lysozyme have been taken to town on their biophysics. The paper I unsuccessfully tried to attach (Potty et al., DOI: 10.1016/j.ijbiomac.2010.12.007) has a fairly clear discussion on the contribution of each thermodynamic parameter.
Best wishes,
Vitor
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