I'm looking at the ability of certain bacteria to solubilize nutrients in soil so I want to test for this property in vitro
I thought I knew this but now am confused as simple as it sounds. I knew that antibiotics are substances of microbial origin which are anti-life of other microorganims.. But I did some reading and...
10 November 2016 6,190 2 View
I am seriously seeking an opportunity/scholarship to do a PhD degree in microbiology in a center of excellence or university outside Kenya . Any links that can help me get such a chance will be...
10 November 2016 2,954 1 View
In the wake of food insecurity and malnutrition all over, is the use of SCP (Single cell protein) gaining popularity or is it still unacceptable to use microorganisms as food? I know microbes like...
06 July 2016 6,114 3 View
Today I watched a very intriguing documentary about a journey into the future of water'. Ant it showed the extremes of climate which have been brought about by continued but gradual degradation of...
05 June 2016 5,391 17 View
Citations help show the value of research and the impacts a researcher or research is creating...Is it allowable for a researcher to self-cite or self-reference his/her work?
07 August 2015 5,565 23 View
Bacterial metagenomics Enzyme identification and extraction
04 May 2015 5,423 1 View
Evolution theory Phylogenetic and phylogenetic trees Phylogenetic models and phylogenetic studies Studies of similarities and differences between organisms
04 May 2015 7,818 4 View
Population diversity studies of bacteria in a water enviroment My major problem is to find the best method for DNA extraction studies so that I can do the sequencing. Is it total environmental DNA...
04 May 2015 8,613 6 View
I have them on Agar slants in the refrigerator. What is my best alternative? And how often do I need to transfer them to fresh medium? I also know that the more you transfer them, then you tend to...
01 January 1970 8,848 17 View
The antimicrobial effect of local anesthetics has been known for more than 50 years, but it still seems to have an unclear mechanism of action. which bacteria? which concentration? etc etc. ....
27 February 2021 4,450 2 View
Hello I'm currently working on a topic related to studying the anaerobic biodegradation of TPH in marine sediments. In this work, sediment should be mixed with fuel oil to a concentration of 20 g...
24 February 2021 9,839 3 View
Hello everyone, For part of my PhD project, I'm hoping to grow some lactobacillus species in the lab so that I can create standard curves for a qPCR quantification of a faecal sample. However, I...
24 February 2021 3,157 6 View
Hello. I want to isotopically label the my protein, and I express it in BL21 RIPL using M9 minimal media. It grows extremely slow. I inoculate the colony from a fresh M9 plate into 5ml M9 medium...
17 February 2021 2,852 2 View
I have been reading various articles and most state that treatment with CaCl2 produces higher efficiency of transformation than MgCl2. Can anyone explain why Ca2+ is better than Mg2+ at...
16 February 2021 8,519 5 View
Hello all, I am interested in deleting a highly expressed non-coding RNA located on a Listeria monocytogenes plasmid. Currently, there are not any optimized gene deletion methods for Listeria...
09 February 2021 4,994 1 View
Dear fellow researchers, Could you please suggest me excellent review papers discussing transcriptional regulations and/or promoter classes in Gram positive bacteria? Thank you so much for your help!
08 February 2021 6,909 3 View
When you use RNAseq I understand that it must follow certain conditions when you use it. One of them is the insertion of a polyA tail. The insertion of the polyA must include then...
05 February 2021 3,770 4 View
I need to assimilate a determining condition for the plant. However, I need to ask you if it is possible to change the properties and concentration of plant nutrients in this material like perlite...
04 February 2021 8,616 11 View
Due to their ubiquitous nature, there is a tendency of the presence of microbes in a given mineral(s). For instance, if microorganisms that love the of pH less than 6 in the pH value, is isolated...
03 February 2021 4,630 10 View