you need this thesis BIOLOGICALLY ACTIVE TRITERPENE SAPONINS
FROM THE ROOT OF THE MEDICINAL PLANT
Polygala senega L. if yes then mail me [email protected]
Following links may be useful for your query....
http://www.ncbi.nlm.nih.gov/pubmed/15030209
http://www.diva-portal.org/smash/get/diva2:610888/FULLTEXT01.pdf
Saponins were extracted using the method developed by Applebaum et al. (1969), slightly modified. The ground material is lipid-free for two hours with 250 ml of pure n-hexane for analysis (reag. ACS Riedel-de Haen). After removal of the organic phase, the resulting precipitate was soaked in 300 ml of absolute ethanol with magnetic stirring at room temperature for 24 hours. The ethanolic phase is evaporated to dryness under vacuum at 40 ° C by rotary evaporator. The dry residue is extracted three times with 100 ml of distilled water / petroleum ether (v: v) heated at 50 ° C in a water bath for 30 minutes. The aqueous phases are mixed and then taken up with 150 ml of n-butanol for analysis (type Riedel-de Haen) for 30 minutes. The organic phase evaporated to dryness at 40 ° C by a rotary evaporator, is weighed and recovery by 1% ethanol for testing.
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