In the anatomical and microscopic observation of the leaf and stem of the plant, can I first take a sample and then proceed to cross-sectioning, staining, and microscopic observation in the following days?
Hello Faride,
for a short period of time, it may be sufficient to store the botanical material in a cool place and protected from dehydration. For subsequent simple morphological or anatomical examinations, storage in 70% ethanol would still be suitable. However, for more demanding histological examinations, storage in a preservative fluid such as AFA would be recommended.(AFA = Alcohol-Formaldehyde-Acetic Acid, be aware of the safety requirements).
With kind regards
Klaus Berkefeld
Dear Klaus Berkefeld Your guidance was very helpful. Thank you.
After collecting the samples, make the cuts and fix them immediately to preserve the plant tissue.