Dear Colleagues,
I would like information about saline aquifers in Brazil. I needed information and maps and I'm having trouble finding public data (wells, depths, salinity, etc.).
If so, could someone please give some insights on the IPTG concentration, the length of the induction period, the temperature during induction, and the cell line that was used. The gene is cloned...
15 August 2023 831 1 View
Hello, I want to silence my gene of interest using ptdT-RNAi vector, so what I need to know is either if the amplicon must include the 5’UTR region, the ATG or both. I know that the length has to...
30 May 2023 307 1 View
I've seen some publications ask for one year before posting elsewhere. A university librarian informed me that no such posting is allowed, on any type of site, including for members only.
20 November 2022 9,144 10 View
Current legislation in Brazil for this type of shipment is very confusing (not to say chaotic) and bureaucratic. I would like to know from colleagues who have had this experience how they did to...
12 November 2021 599 8 View
I collected a seaweed sample from the Kenyan coast (Mkomani). I was looking for Caulerpa macroalgae. I am uncertain of the specific name of the pictured seaweed. Can you help me identify the species?
20 October 2021 7,530 7 View
Hello, I am doing a microsatellite PCR and the bands appear with a few definition, the electrophoresis conditions are the following, TBE buffer 0.5X pH 8.4 (according to Sambrook, Molecular...
29 August 2021 2,860 3 View
I am monitoring the degradation of contaminants using immobilized enzymes on carbon support. However, to complete the mass balance of the degraded reactant, I have to perform a mass balance on...
06 April 2021 6,802 2 View
I am struggling on how to calculate this. I know I need the MW, but I am not sure how these all tie together.
01 April 2021 4,795 1 View
Hello everyone, I did a transformation of E. coli DH10B using pUC18 plasmid. The bacterias did grow on MKL medium with ampicillin but the colonies showed a white/pink colour and the medium was...
08 February 2021 10,086 3 View
Hello, I am not a biology researcher, but I am working in intradisciplinary research and would like to know the answer to this question, which I have not being able to understand. This questions...
06 January 2021 4,195 4 View
For example, if low-density polyethylene (LDPE) is equilibrated with PCB contaminated marine and freshwater sediments, can LDPE-lipid partition coefficients be used to obtain equilibrium...
28 July 2024 263 1 View
In order to test different water parameters I need to know how to convert conductivity to Salinity and TDS in ppm or ppt. Give me the reference please.
27 July 2024 2,414 2 View
i compare between the antioxidant and oxidative stress level between negative control rats (saline) and CCL4 intoxicated rats. i found the level of GSH and TAC is more in toxic group than normal....
26 July 2024 1,887 0 View
If you understand what is the necessary and sufficient condition for exism movements like Brexism, Trumpism, Brazilianism.... to persist at all cost in power once they have power are within a...
10 July 2024 8,048 1 View
Overview of Normal Saline in Fluid Therapy: Mechanisms, and ... How can ongoing education and team-basedsimulations improve the effective use of normalsaline in clinical practice?
27 June 2024 3,972 1 View
Overview of Normal Saline in Fluid Therapy: Mechanisms, and ... What roles do different healthcare professionals(physicians, nurses, pharmacists) play in themanagement of normal saline therapy?
27 June 2024 848 1 View
Overview of Normal Saline in Fluid Therapy: Mechanisms, and ... How does normal saline differ from other crystalloidsolutions in terms of composition and clinicalapplication?
27 June 2024 4,235 1 View
Overview of Normal Saline in Fluid Therapy: Mechanisms, and ... Why is it important to avoid or carefully managenormal saline use in patients with renal impairment orcongestive heart failure?
27 June 2024 7,469 1 View
Overview of Normal Saline in Fluid Therapy: Mechanisms, and ... What training should healthcare professionals receiveto ensure the safe administration of normal saline?
27 June 2024 2,934 2 View
Overview of Normal Saline in Fluid Therapy: Mechanisms, and ... In what situations might you choose an alternative tonormal saline for fluid resuscitation, and why?
27 June 2024 3,270 2 View