What are the requirements to place the order for CRISPR/Cas9 plasmids?

More Anshika Srivastava's questions See All

What are the steps for cloning a gene fragment in the pGEX-4T1 vector?

My DNA insert size is 746bp. I have designed the primers with the EcoR1 and Sal1 enzyme sites at 5'.

11 December 2013 7,462 5 View

How can one design the Cas9 gRNA for carrying out CRISPR/Cas9 mouse genome editing?

I just narrowed it down to the specific exon of gene of my interest but I am not sure how to make multiple gRNAs for the same gene target? What points should be kept in mind while designing the gRNAs?

11 December 2013 6,058 7 View

How to amplify a gene of 6.9 kb to clone in pDONR221 using Qiagen kit for reaction mix?

I used initial denaturation at 95 for 10 min followed by 94 for 45 sec. Temperature gradient for annealing temp from 62 to 73 at 1 min. Extension time : 68 for 6 min and further 68 for 10 min....

11 December 2013 3,048 28 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

16 June 2021 0 0 View

fatal error

16 June 2021 0 0 View

fatal error

16 June 2021 0 0 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

How to repeat an operation on an atom selection for multiple times in PyMol?

Hi, I am trying to construct a multi-layer fibril structure from a single layer in PyMol by translating the layer along the fibril axis. For now, I am able to use the Translate command in PyMol...

02 March 2021 4,569 4 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Does anyone have a recommendation for yeast reporter gene plasmid/protocol?

Hi, could anyone recommend a plasmid and/or protocol for reporter gene assay in S. cerevisiae? I want to assess the effect of growth conditions on a transcription factor, so I want to clone it`s...

01 March 2021 210 1 View