Amna Rafiq

4 Questions 3 Answers 0 Followers

Questions related from Amna Rafiq

What are the reasons to get two bands on Western Blotting?

Hi! everyone. I need someone help to understand wb results. I got two band of different sizes, actually it is the sum of my actual band. Is there any possibility that my protein has been split or...

04 January 2024 2,942 2 View

Why bands size is different during single digestion with rong band,?

Size of my gene of interest cloned into pet 28 alpha 6857bp. After transformation I did plasmid digestion with ingle enzyme xhoI. But the single band size is not equal to total size 6857 instead...

15 January 2023 6,534 3 View

What should be done if there is a problem in double digestion band location on different sites instead of exact location?

As Salam O Alaikum! I am working on restrictions analysis after transformation in DH5 alpha cells. Bands for Transformation was on exact location. But for double and single digestion bands are at...

14 January 2023 5,388 0 View

Please anyone tell be that which one is the best tool for #d protein Model Prediction without template, except Robbeta, Phyre2, Rosetta, Raptor x?

Hi! I need a denovo protein 3D model prediction tool for my own protein construct.

03 December 2022 9,799 4 View