Why do cells die after adding MTT dye?

I was doing MTT assay, and in the last day before adding the MTT; the cells looked good (or so I think), but after aspiration of the drug and addition of MTT dye I noticed that most cells seemed...

07 February 2021 3,846 4 View

How can I stain 3d printed cells in hydrogel without damaging the gel?

I tried staining my cells in a gel using standard protocols with 4PF% for fixation and triton-X or saponin for permeabilization but the gel always gets damaged throughout the steps, and at the end...

17 January 2021 4,170 1 View

Why do I get low absorbance in MTT assay?

I have been trying to do MTT assay using HCT-116 cells but I have been getting very low absorbance readings (absorbance is usually 0.25 and below) even though iam seeding 30,000 cell/ well. I have...

16 November 2020 3,588 3 View

Can somedbody advice on how to deal with various lingering covid-19 symptoms especialpersistent tiredness?

I contracted the infection in mid May.My symptoms included runny nose,skin,widspread skin tinglings,slight headach,profuse sweating,and extreme tiredness that I was not able to walk for few meters...

12 September 2020 2,385 4 View

I am new to cell culture technique and reading papers on cell culture.Is there any criteria for selecting certain cell culture (cell line)?

I need to select a cell type to start my experiment. My experiment is on female genital tract infection and I intend to see cytokine expression. I have read about J774 but I do not know why to use...

09 August 2020 8,504 3 View

Need help- how to use primary and secondary data in same paper in regression?

Actually am trying to use regression for my paper where am drawing primary data questionnaire(independant)from few universities students and trying to establish relations with student...

02 May 2020 7,097 3 View

Can anyone help me convert the concentration of human anti-hepatitis B antibody (Hepabig) from IU/ml to mg/ml?

I am using Hepabig brand which only states 100 IU/0.5 ml concentration of antibody but no mg/ml immunoglobulin concentration estimate.

05 March 2019 5,808 15 View

How to tell apart primordial germ cells from EG cells?

Is there any method that can tell apart EG cells from primordial germ cells? By morphology or surface marker, gene expression, et cetera. ?

11 November 2018 2,117 3 View

How to test purity of cultured macrophages with flow cytometry?

What's a good strategy to gate macrophages cultured from isolated bone marrow cells? Available antibodies are CD115, CD11b, F4/80 and Gr-1.

31 October 2018 1,529 2 View

Is there a simple method to use pslegend (GMT) to create a legend for 2-D plot?

I am new for Generic Mapping Tool (GMT), but I would like to work through a few simple examples of generating legends (ie. multiple points or lines plotted on a single figure using psxy). In the...

26 April 2018 8,197 3 View

error in running a model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

error in modelling model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

MGL tools

20 June 2021 0 0 View

How climate change will influences human and animal behavioral change?

The role of climate change influencing the human behavior as well as economics interest

01 June 2021 0 0 View

flexibility of polymers chain

effect of double bond on the flexibility of polymers

07 April 2021 0 0 View

i have some problem with chtMultiRegionSimpleFoam

--> FOAM FATAL ERROR: Maximum number of iterations exceeded From function Foam::scalar Foam::species::thermo::T(Foam::scalar, Foam::scalar, Foam::scalar, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar) const) const [with Thermo = Foam::hConstThermo >; Type = Foam::sensibleEnthalpy; Foam::scalar = double; Foam::species::thermo = Foam::species::thermo >, Foam::sensibleEnthalpy>] in file /home/ubuntu/OpenFOAM/OpenFOAM-4.1/src/thermophysicalModels/specie/lnInclude/thermoI.H at line 66. FOAM aborting #0 Foam::error::printStack(Foam::Ostream&) at ??:? #1 Foam::error::abort() at ??:? #2 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::calculate() at ??:? #3 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::correct() at ??:? #4 ? at ??:? #5 __libc_start_main in "/lib/x86_64-linux-gnu/libc.so.6" #6 ? at ??:?

24 March 2021 0 0 View

Formulation of polymer color flakes

I am writing to request some information for making polymer color flakes. I would appreciate the help.

17 March 2021 0 0 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View