What are the potential risks involved with the release of genetically modified organisms (GMOs) in the soil?

More Bruno Borsari's questions See All

What are the latest technologies to detect with accuracy when cows are approaching ovulation, in order to maximize fertility and conception rates?

Estrus detection is very important to enhance fertility in livestock. I am interested in learning new technologies/approaches that maximize the conception and fertility of bovine species.

31 December 2018 8,392 15 View

How different are the communities of invertebrates in tropical soils?

I am interested in learning more about the abundance and diversity of soil invertebrates. As tropical zones show high diversity above ground, I wonder whether the same is reflected by the...

11 December 2018 7,486 3 View

How effective is meliponiculture (raising of stingless bees) among rural communities of central and south America?

Meliponiculture is the cultivation/raising of stingless bees species. These are native to central and south America and in many indigenous communities they have been kept/managed for millennia. I...

07 August 2018 4,766 4 View

What ethic principles/values can be employed to achieve a sustainable development?

Too often the discussion around the concept of sustainability is linked to the environment, the economy and the social issues however achieving a sustainable development must be supported by...

03 April 2018 7,776 7 View

What is the impact of urban agriculture on the global scale?

Food production out-puts from cities can be significant and since urbanization is on the rise worldwide I would like to learn more specifically what is/are the impact/s of growing food in cities.

03 April 2018 3,376 4 View

What is the efficacy of liquid biofertilizers (e.g.: compost tea) in the production of fruits and vegetables?

What fruit and vegetables plant species respond best to the application of biofertilizers like compost tea?

02 March 2018 6,438 25 View

What can be suggested to design farms so that mutualism between animals and their plant partners may increase efficacy to foster sustainability?

What can be suggested to design farms so that mutualism between animals and their plant partners may increase efficacy to enhance the sustainability of food production systems?

02 March 2018 8,830 3 View

Is there a cheap method to assess the presence of saprophytic fungi in soils?

How can soil fungi and ectomycorrhyzae be detected in the soil? Is there a simple method that can be used to demonstrate their presence when you are in the field with farmers?

02 March 2018 8,627 6 View

How is plant nutrition being affected since the widespread use of synthetic fertilizers?

How is plant nutrition being affected since the widespread use of highly soluble synthetic fertilizers to more complex, organic soil amendments, that are typically applied on organic farms?

02 March 2018 617 14 View

What are key elements that will determine the time for a degraded soil to be restored?

Inevitably any farming activity disturbs the soil and with it, soil organic matter (SOM) will decrease, or even be lost completely (e.g.: desertification). How long will it take to restore a soil...

02 March 2018 661 19 View

How do I increase the yield of my PCR product?

Hello, We would like to increase the yield of our PCR product. We are running a series of PCR reactions that is targeting ~1.1kb sequence. We begin each reaction with ~400pg of template DNA...

02 March 2021 4,029 3 View

Research on Supply Chain Management in Indian context?

Hi, I am planning to apply for the PhD degree in the Supply Chain Mgt. with specific area of "Cold Storage warehouses" during Pandemics and wars. Where lock downs and shut downs are frequent....

02 March 2021 285 2 View

PACYC PCR not working after several attempts. Would anyone have any suggestions?

So, I have been trying to run a pACYC PCR which will be used later on for a Gibson Assembly. However the PCR is not working. I have already tried gradient PCR and changing extension time; however...

02 March 2021 1,146 2 View

How to address this issue in the analysis of Real-Time PCR results?

To dear Researchers, I was analyzing a series of concentration for estimation of Real-Time PCR efficiency. The concentration was 1:10. I used MS-excel to evaluate Slope. The result of slope was -8...

01 March 2021 8,683 4 View

Runs of nucleotides in Sanger sequencing?

I performed site directed mutagenesis, transformation, and then I sent out plasmids for Sanger sequencing and found out that there is extension of DNA just before the stop codon. I am not sure...

27 February 2021 547 3 View

Can thermal cycler machines be problematic during PCR?

Hi, my question is about the heating of thermal cycler machines and I hope some of you had experienced a similar thing previously. There are two thermal cycler machines in the lab(BioRad) and for...

26 February 2021 4,777 4 View

Calculating volume of DNA required from DNA concentration?

Hi I am a bit confused. They are asking me to find out the volume of DNA required in ul (a total of 30-100 ng for genomic DNA) from the DNA concentration in the nanodrop reading which was 404.8...

26 February 2021 5,029 2 View

Is anyone working on a project focused on immigrants/newcomers working in the hospitality industry and how they have been impacted by COVID?

The on-going Covid-19 pandemic has had devastating economic effects on many industries. However, few have suffered to the same extent as full-service restaurants. Numerous studies have shown that...

24 February 2021 9,496 4 View

What is the optimum time and temperature of blanching and pre-frying potato for frozen fries?

I am helping a local home industry for developing frozen fries from their local farm. I want to know what is the optimum blanching and pre-frying temperature and time for processing. Please also...

23 February 2021 1,616 5 View

Could not amplify CD28 and CD3z ectodomain from cDNA of Jurkat Cells ?

For my experiments, I have to express ectodomains of human CD28 and CD3z. As a source, I extracted RNA from Jurkat cells and verified the quality of RNA on the gel. I, then, prepared cDNA using...

23 February 2021 7,051 4 View

Yuan-Yeu Yau

It was reports, in some cases, it would affect soil bacteria. See 2015 article below:

Article Belowground environmental effects of transgenic crops: A soi...

"Plants modified to express phytopathogen/phytoparasite resistance, or traits beneficial to food industries and consumers, differentially affected soil microorganisms depending on transformation events."

M. C. Sidhu

There are number of reports both in favor of GMO and others questioning the GMO. I mean mixed response is there. As far as the "Transgenic DNA" is concerned, it has also been reported in the non-GMO crops also which is generally called natural transgenic.