In my experience, Fusarium genus usually sporulate macroconia in common media (e.g. PDA or MEA).

If you're asking it,probably it means that it doesn't work with your strains.

You can use a medium made by tomato plant tissues.

It's very easy to do by autoclaving the plant tissues, followed by well drying. Put your sterilized tissues into a petri dish containing WA (water-agar) and put a mycelium plug on it, then wait for the formation of conidiophores.

If it doesn't work, try to use a standard medium and "play" with temperatures (maybe it work some hours at 30-35-40°).

Just as a curiosity, try to put some plates in microwave for 5 seconds at 600W and check your colonies in few days. I remember it accidentally worked with some fungi (and it works to recover some cultures contaminated by mites, when you have not a backup of your strain, personal experience).

Hello, several years ago, when I worked with Fusarium oxysporum, I use Corn Meal Agar to produce macroconidia, and It was very effective, I hope that this tip will be usefull for you.

Light can influence sporulation of many Fusarium species. A light/dark cycle is recommended for production of macroconidia, particularly in sporodochia. In addition, exposure to actual or artificial daylight or near UV can enhance sporulation.

A lower nutrient medium with some plant material is generally better for macroconida production and is less likely than PDA to lead to loss of conidiation or other aberant growth. We have had good results with a half-strength V8-juice agar with a diurnal light/dark cycle for our F. oxysporum.

You may try using ph adjustment

Provide good CN ratio in media, expose the culture to minimum 16hrs of white light.

You could try Bilay's medium modified by Joffe:

1 g potassium dihydrogen phosphate (KH2PO4)

1 g potassium nitrate (KNO3)

0.5 g magnesium sulphate (MgSO4.7H2O)

0.5 g potassium chloride (KCl)

0.2 g starch powder

0.2 g glucose

0.2 g sucrose

15 g agar

1 litre distilled water

Autoclaving for 20 minutes at 120°C.

Strips of pure cellulose lens paper are aded before the agar had set.

Sources:

The Genus Fusarium, C. Booth, Comonwealth Mycological Institute, Kew, Surey, England, 1971

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