Any easy method of analyzing vitamin D in foods by HPLC?

Looking for method of extracting and analyzing vitamin D in foods by HPLC

24 February 2021 7,524 7 View

Which aqueous solution should I test to make positive NPs stable to get mayor zeta potential +40 mV?

Hi, I need to find the correct media to make my positive charge NPs stable in an aqueous media. I have tried different solutions at different pH but the zeta potential does not increase more than...

21 January 2021 6,490 6 View

Can I use G418 for selection of HEK 293 cell lines that already have a plasmid with kan and Hygro integrated?

I am doing a kill curve in HEK 293 cells with G418(0-1000ug/mL) and they don't die. They were previously transfected and selected with hygromycin and are integrated with a plasmid has also...

05 December 2020 3,232 3 View

Lactophenol Cotton Blue with benzyl alcohol?

In the preparation of a Lactophenol Cotton Blue, can phenol be substituted by benzul alcohol? (Question for some students in a laboratory lacking some reagents.)

03 December 2020 8,621 1 View

Does anybody know where to download codecs for The Observer XT11 and or XT13?

I am trying to observe mp4 videos using Observer. Videos run with no problem on VLC and BORIS animal behaviour software, but Observer 11 and 13 don't want to run the videos as it says codec...

06 July 2018 3,416 0 View

M1 or M2 polarization in BMDmacrophages?

I want to induce M1 and M2 polarization in bone marrow-derived macrophages but I've got many problems with this. I'd like to use CD206 as a marker of M2 macrophages but I do not see any...

01 February 2018 8,920 2 View

Annealing and extending oligos

Anybody has a working protocol to anneal and extend ss oligos to get a dsDNA using Taq DNA polymerase?

11 May 2017 818 4 View

Can anybody familiar with "distance-based Moran's eigenvector map analysis" provide advice on how to do it? Also any suggestion for software on this?

Recently, studies have demonstrated that the use of Mantel test is not corrent when detecting spatial structure or spatial correlation between two multivariable data sets (community structure and...

30 May 2016 9,790 7 View

Is it Sustainability a motivational factor in the field of HR Management?

I am working in this issue and would like to have a feedback of what experts think of it.

08 September 2015 4,319 3 View

Is anybody familiar with duplexing complementary sequences of DNA?

I have two complementary sequences of DNA and I need to form a duplex. Each sequence have 61 nuecleotides. Can you help with a protocol? Thank you.

19 March 2015 9,159 4 View

Polymer composites suddenly solidifies?

I am making polymer composite by solvent mixing method. When I am trying to add filler solution in polymer solution it quickly get solidified. How to make them then uinform? It doesn't give time...

03 March 2021 7,540 4 View

What solvent can I used for Paal Knorr reaction between 6-amino hexanoic acid and 2.5 hexanedione?

I am try to make the Paal Knorr reaction between 2.5 hexnedione and 6-amino hexanoic acid, my problem is type of solvent to use, because 6-hexaminohexanoic acid is soluble in water but I am not...

02 March 2021 4,443 2 View

Should we change media after1st 6hrs after plating HUVEC bought from INVITROGEN?it's not recomended by invitrogen.Will DMSO present in stock matter?

We have HUVEC bought from Invitrogen. Cat no -C-003-5c. while first plating the cells we have bought the protocol suggests not to centrifuge the cell with media to remove the DMSO. Should we...

02 March 2021 3,713 2 View

How to polymerize TEOS?

I want to polymerize TEOS on a surface. I know that NH4OH is a preferred catalyst and an alcoholic solvent should be used .But I can not find certain amounts of each material to use. Does any one...

02 March 2021 1,404 6 View

How can extract ciprofloxacin hydrochloride disolve in water er to organic solvent such as dichloromethan or any other solvent?

I need a procedure to extract the ciprofloxacin hydrochloride from aqueous solution by using dichloromethane in detail,. I thank you very much.

01 March 2021 4,306 1 View

How to separate water from glycerol solvent in a solvent recovery vacuum distillation column?

I am currently doing my third year design project. I have been assigned to design a glycerol recovery column in vinyl acetate monomer production. The process unit before the recovery column is an...

01 March 2021 9,525 4 View

How to make fractions of chloroform extract of plant, using seperating funnel?

28 February 2021 8,466 2 View

Bought HUVEC from Invitrgen.need to revive it.expected it is cryofreezed in DMSO.Do I need to remove the DMSO for plating the cell for the 1st time?

After I cryofreeze the cell do I need to remove the DMSO for culturing the cell for next passages .

28 February 2021 7,738 3 View

POlymer+DMSO solution and moisture??

What could be the reason of moisture at the sides of Vial when I am preparing my polmyer+DMSO solution for membrane fabrication? What etiquettes I am possibly missing here?

28 February 2021 6,516 5 View

Why am i not getting solid after reacting APTES with salicylaldehyde?

The reaction of APTES with salicylaldehyde in ethanol leads to the formation of imine compound yellow solid which is mention in literature, but I am not getting yellow solid I don't know why? when...

28 February 2021 2,955 2 View