I have a problem in Vibrio harveyi survival in sea water, what are the water parameters needed for vibrio harveyi to survive in water?
The temperature, pH and salinity of seawater must be 20oC, 7 and %2-3.
My data for fungicide treated replication shows higher RMSE and R-square but less CV compared to the untreated replication for a regression between vegetation indices and yield. How correct is it?...
23 November 2020 2,079 6 View
Hi All, I am working on developing a computational model to find protein coding and non-coding regions of COVID-19 DNA sequences. To test my model, I need CDS information of COVID-19 DNA...
30 April 2020 4,162 7 View
Hi, Can anyone please explain me how to use Genscan tool to find exons and introns for a given gene sequence? I want to calculate sensitivity and specificity to check its performance for a given...
30 December 2019 2,083 3 View
I am studying water splitting on Pt substrates. Is it necessary to use a reference electrode or is it possible to study the system using a two electrode setup where the Pt counter electrode is...
19 November 2019 7,182 3 View
I have a huge data for which I want to apply wavelet and plot scalogram. the below code works for small data. However, I have a huge signal, for which I am not able to plot scalogram. Can anyone...
10 April 2019 1,959 3 View
I am trying to process the wheat crop data collected using ASD for reflectance. However, I see two different options in ViewSpecPro software- Absolute reflectance and reflectance(transmittance)....
25 March 2019 1,488 4 View
The organism is Streptomyces but the exact strain is not known and hence the sequence. So after sending the PCR product for sequencing we have the part of the whole sequence that contains the gene...
26 February 2019 3,182 4 View
I had 15 genotypes of sorghum planted in 6 replications. There was pre-harvest grain yield loss by birds that made it difficult to compare these genotypes based on yield performance. Is there any...
20 December 2018 2,294 3 View
I don't see nitrogen in the oxidizer(air). Should I consider nitrogen while calculating mass fractions?
08 September 2018 7,522 1 View
I'm trying to simulate premixed combustion in fluent. I'm using methane-air mixture for combustion with a 2 step reduced mechanism. I'm confused about the mass fraction calculations.
01 September 2018 8,371 3 View
Good afternoon, I recently used OmniLog from BIOLOG for my experimentations : I tested the metabolism of different strains on 2 types of plates. I have 16 strains of 3 different groups...
02 March 2021 3,584 1 View
Hi everyone, Illumina provides a list of primers to amplify with high taxonomic coverage the ITS1 region for further fungal sequencing, but I cannot find the exact amount necessary of each...
25 February 2021 6,969 3 View
Warm greetings My name is Ayichew S. and I am doing my Ph.D. in Medical Microbiology (on Human papillomavirus). At this stage, I have almost completed a clinical sample Cervical swabs)...
25 February 2021 1,660 3 View
Warm greetings! My name is Ayichew S. and I am doing my Ph.D. in Medical Microbiology focusing on Human papillomavirus). At this stage, I have almost completed a clinical sample (Cervical swabs)...
25 February 2021 3,612 3 View
Hello everyone, For part of my PhD project, I'm hoping to grow some lactobacillus species in the lab so that I can create standard curves for a qPCR quantification of a faecal sample. However, I...
24 February 2021 3,157 6 View
I am aware of a study that has compared the use of citrated and defibrinated sheep's blood in microbiological media (Hair Sheep Blood, Citrated or Defibrinated, Fulfills All Req... ), but can...
10 February 2021 5,927 1 View
I will be using the lowered pH broth for MIC test of some disinfectants, and I do not want the acid to have any interfernece with MIC results.
03 February 2021 6,959 4 View
Due to their ubiquitous nature, there is a tendency of the presence of microbes in a given mineral(s). For instance, if microorganisms that love the of pH less than 6 in the pH value, is isolated...
03 February 2021 4,630 10 View
Hi all, How would you use qPCR to quantify the amount of a particular bacterial species in a sample experimentally? I understand you could use specific primers for the 16s V region of a...
01 February 2021 7,153 4 View
How can I know the stationary phase of growth of several bacteria? We are aiming to collect exoproteome of several bacteria. We know that the ideal time to collect exoproteome is during the...
31 January 2021 4,528 10 View