Krishan Kumar
The organic solvents most commonly used for extraction of mycotoxins are:chioroform, acetonitrile, methanol and acetone. These solvents are mixed with a given ratio of a more polar solvent (water, dilute acid, aqueous solution of salts) to aid the breaking of weak electrostatic bonds which bind some mycotoxins to other substrate molecules (eg proteins).
The ground sample, or preferably an aqueous slurry, is either shaken with the extraction solvent for 30-45 minutes or blended at high speed for about 3 minutes. It should be noted that an explosion proof blender is recommended for use with inflammable solvents such as acetone and methanol.
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