I was performing wound healing assay seeding 300,000cells/well in 6 well plates with 2mL RPMI medium.
At 0hr after intervention, the cells looked like the first picture.
At 24hr after intervention, the cells looked like the second picture
at 42hr after intervention, the cells looked like the third picture.
The control (DMSO) group on the other hand, demonstrated morphologies of the fourth, fifth, and sixth pictures at 0hr, 24hr, and 42hr after intervention respectively.
The thing is, the duration of this entire transformation shortened to about 20~24hrs when I redid the experiment under the same conditions, but with 1mL medium (same amount of cells seeded, same drug concentration, same type of 6 well plate, just with half the medium)
We can clearly see morphological differences at 42hr (the third vs the sixth picture) between the intervention and control groups, and I'm curious as to what may be the cause of it.
BTW, cell cycle analysis via flow cytometry was also performed, and there doesn't seem to be much of a difference between the control and intervention groups, with no signs of increase in SubG1 upon intervention, which kind of dismisses apoptosis. I'm therefore suspecting the possibility of autophagy.
So does anyone know the morphological changes of PC3 cells during the process of autophagy? Or does this indicate something else going on that I haven't considered?
THANX!!! :)
Hello, Evra!
You see this article, please, there is a morphology of PC3 cells
Article Cytotoxic Effects of the Ethanol Bane Skin Extract in Human ...
you can take a look to this articles:
https://www.jscimedcentral.com/Autoimmunity/autoimmunity-4-1014.pdf
https://core.ac.uk/download/pdf/287193172.pdf
- Badges
- Science topic
- Similar topics
- Agriculture
- Horticulture
- Seeds