Or which model cell line triggers the apoptosis upon exposure of natural (anticancer) compounds for the study of anticancer property of same? And what will be the control model cell line for comparative differentiation for anticancer property of same? How within it we can observe the initiation of repair mechanism in diseased pathway?
Usually the anticancer activity of a molecule is studied on series of cancer cell lines. Firstly you have to test your molecules on different type of cancer cell lines like Leukemia (K-562, MOLT-4, REH, Nalm-6 etc.), breast cancer (MCF-7, MDA-MB etc.), cervical (Hela), Lung (A549, A-427), kidney (A498) and so on. Along with these you should also use a normal (non-cancer) cell line like fibroblast or other tissue cell lines to test the effect of your molecules on normal cells.
You can get the details of all the cell lines in ATCC website.
Hope it helps.
All the best.
-Rohit
You can use cell lines like liver (HEP-2), lung (A-549) , colon (Colo-205), breast (MCF-7), prostate (PC3 & DU145), ovary (IGR-OV-1), neuroblastoma (IMR-32), leukemia (THP-1), acute lymphoblastic leukemia (HL-60) etc. for your experiment depending on the availability. Various assays like SRB assay or MTT assay for cytotoxicity and antiproliferative activity can be performed.
Hi,
as cancer is not one disease but a multitude of different diseases with different origins and mechanisms of disease there is not one good cancer model. If you have a certein compound in mind that you want to test for it's anti-cancer properties, you should maybe go for the nci 60 cancer cell line panel -which you can google and buy. that will cover a lot of commkon cancer and corresponding cell lines. I copied an abstract from nat. rev. cancer for you:
"The NCI60 human tumour cell line anticancer drug screen
Robert H. Shoemaker1
Abstract
The US National Cancer Institute (NCI) 60 human tumour cell line anticancer drug screen (NCI60) was developed in the late 1980s as an in vitro drug-discovery tool intended to supplant the use of transplantable animal tumours in anticancer drug screening. This screening model was rapidly recognized as a rich source of information about the mechanisms of growth inhibition and tumour-cell kill. Recently, its role has changed to that of a service screen supporting the cancer research community. Here I review the development, use and productivity of the screen, highlighting several outcomes that have contributed to advances in cancer chemotherapy."
You can use different leukemia cell lines. MTT assay is widely used a simple method which allows us to analyze cell viability and determine the values of IC 50 for different anticancer agents
Thank you all for valuable information. Can someone add further information related to the types and names of apoptosis resistant, apoptosis sensitive and multi-drug resistant (MDR) cancer cell lines. I am interested to study differential behaviors of these cell lines against some natural compounds.
Thank You.
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