What are the methods to evaluation in-vitro anti inflammatory activity of plant extracts?
Article A Therapeutic Review on Aloe Vera - A Traditional Phytomedicine
Aloe - Vera : Anti-inflammatory & Wound Healing .
Several assay systems and in vitro methods have been frequently used to assess the anti-inflammatory effects
of herbal extracts.These include 1-Protein denaturation assays 2-Membrane stabilization
assays 3-Assay of cyclooxygenase and 5-lipooxygenase inhibition.
4-Assay of proteinase inhibition.
5-Hyaluronidase inhibition assay.
6-Rat paw edema modeling.
Dear Ali
this can be useful.
*lnhibition of albumin denaturation*
Assessment of invitro anti-
inflammatory activity
Inhibition of albumin denaturation
Method of Mizushima et al 25 was
followed with minor modifications. The
reaction mixture was consisting of test
extract at different concentrations and
1% aqueous solution of bovine albumin
fraction. pH of the reaction mixture was
adjusted using small amount of 1N HCl.
The samples were incubated at 37oC for
20 min and then heated at 57oC for 20
min. After cooling the samples, the
turbidity was measured
spectrophotometrically at 660 nm. The
experiment was performed in triplicate.
Percent inhibition of protein
denaturation was calculated as follows:
Percentage inhibition = (Abscontrol –
Abssample) X 100/ Abscontrol
Sachin S et al., 2009. Check the full paper as attached.
The most used test to evualate the in vitro anti-inflammatory activity, I think it's the 15-Lipoxygenase test. I suggest you to see the articles of Bouajila et al.
Article A Therapeutic Review on Aloe Vera - A Traditional Phytomedicine
Aloe - Vera : Anti-inflammatory & Wound Healing .
Protein denaturation assays and membrane stabilization assays are frequently used to evaluate in vitro anti-inflammatory activities. Many researchers have used a minimum of three in vitro assays to evaluate the anti-inflammatory activity of herbal constituents.
http://www.ijddr.in/drug-development/invitro-antiinflammatory-activity-of-methanol-extractof-enicostemma-axillare.pdf
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4160675/
Article In vitro assays to investigate the anti-inflammatory activit...
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