4% PFA.

Hi, Hussein.

1) Perfusion fixation with 4% PFA in PBS through heart, followed by OCT cryostat procedure for analyses by IF, In situ hybridization

2) Perfused PBS-DEPC and snap freezing in LN2, followed by storage -70C to preserve tissue mRNA for molecular studies.

3) Isolate brain and snap freezing in LN2, followed by storage at -70C to preserve tissue proteins and genome for biochemical and epigenetic studies, respectively.

4) CLARITY technique for IF or GFP/CFP/RFP/YFP transgenic mice for nerve networks studies.

Regards.

Thank you so much Mr. Tedla and Mr.Lee for your responding

Heartiest regards