What are the limitations of Lupus anticoagulant assays?

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Dose of adalimumab in GI involvement of DADA2?

The dose of adalimumab used in rheumatology practice is different than that used in IBD. What is the biological basis of use of higher dose in IBD and does it hold true for treatment of GI...

31 December 2018 4,615 0 View

Protocol for DNA extraction with whatmann 3mm paper ?

Can someone share the protocol and experience of DA extraction with whatmann 3mm paper

10 November 2018 9,264 0 View

What are the recent recommendations of reporting APLA tests?

need clarity on interpretation of tests

01 February 2018 5,766 0 View

What happens to ADA 2 activity levels when the patient is treated with anti tnfs? Do they come up on treatment?

If someone has been started on anti tnf suspecting DADA2 then would doing ADA2 enzyme activity help?

01 February 2018 635 0 View

Are there any published reports of ADA 2 deficient PAN(DADA2) from Asia and if there are then what have been the treatment outcomes

This may help in knowing about the experience of researchers from asia in dealing with this disorder.

05 June 2017 1,278 0 View

Does Anyone have expertise in in vitro transcription and RNA pull down assay?

I am currently working on LncRNA; to know the lncRNA-protein interactions I want to do RNA pull down assay, so I need to design primers with T7 promoter. I need assistance in this regard.

07 August 2024 6,622 1 View

How to improve plaques in Double Layer Agar Assay?

Hi all! My thesis groupmates and I are working on purifying an E. coli bacteriophage. The phage was handed down to us by the previous thesis group, but they only managed to purify it to about...

06 August 2024 5,801 4 View

Are there any commercially available Donkey anti-Alpaca secondary antibodies?

Are there any fluorescently labeled anti-Alpaca secondary antibodies raised in Donkey? So far I have only been able to find anti-Alpaca secondaries raised in Goat. Or is this not possible due to...

04 August 2024 4,255 1 View

The use of data from PubChem for commercial purposes?

Hi, I'm curious to know if data on chemical compounds from PubChem, such as water solubility properties, can be used to train a machine learning model for commercial purposes. Will this infringe...

30 July 2024 8,707 1 View

How do we pick data for determination of Validation Acceptance Criteria?

Hello, colleagues! There is commenting open for new upcoming edition of USP 1033. Validation target acceptance criteria is now different from what it used to be and it doesn't include Cpm....

23 July 2024 7,292 3 View

I'm conducting a study on Research Commercialization in Pakistani HEIs and would appreciate your participation in a brief survey?

Dear Participants- Faculty and Researchers from Pakistani Higher Education Institutions, Thank you for participating in research study that aims to 'Analyze Individual and Institutional Factors...

18 July 2024 7,024 1 View

Can anyone suggest the specification of the test tube used for the tube adherence method for biofilm assay?

when we grow biofilm in test tube, the pipette often do not reach near the content. so we have to decane it directly or use a smaller test tube.

17 July 2024 3,532 0 View

Trouble finding an anti-Digoxigenin antibody to perform Southern Blot technique?

I'm currently using Roche anti-DIG-AKP Fab fragments to perform Southern Blot assays, but this reagent is delayed since november last year. It's anybody facing the same problem? If so, how are you...

15 July 2024 6,668 0 View

Techniques regarding protein-protein interaction in vitro?

What techniques or assays will be used when a purified lyophilized powder is purchased from a company to study protein-protein interaction?

15 July 2024 1,598 3 View

What would you do next?

Studying a specific monogenetic disease based on mutations in different individual genes. I try to recreate the disease phenotypes of donors in vitro. I knocked out a gene x with above 90%...

12 July 2024 6,313 0 View