I want to find out one candidate that is regulating another one either at the translational level? What are the experiments I can perform to prove this?
Dear Debasish, depending on the organism/protein/... you are working, completely different approaches are suggested. Please give the details can help us to help you... Anyway your question is flagged with "methods" only, please find 4 more specific topics, will fit the specialists can resolve your problem.
You could compare transcriptional and translational levels. Reduced or enhanced proteins levels in regions of overlapping expression would suggest a regulatory interaction, with the caveat that other regulatory genes may act as intermediates, that the proteins may be transported etc., etc.
If the candidate regulator has no opportunity to interact with the candidate target before translation then you might have to change your hypothesis.
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