Heparin (a mucopolysaccharide) severely inhibits PCR.  It is sometimes difficult to do PCR on plant material on plants unless you add CTAB (cetyltrimethyl ammonium bromide) to the buffer to remove polysaccharides and other interfering substances (you can look up a protocol on the internet).  I once know a person who was trying to do PCR from snails (which produce lots of mucous, and hence, mucopolysaccharides), and had trouble until he added CTAB to the extraction buffer.  If you are having trouble with PCR using  DNA from plants or organisms with lots of mucous, you could try adding CTAB to your extraction buffer.  Otherwise, I do not think most carbohydrates have much effect on PCR.

Heparin (a mucopolysaccharide) severely inhibits PCR.  It is sometimes difficult to do PCR on plant material on plants unless you add CTAB (cetyltrimethyl ammonium bromide) to the buffer to remove polysaccharides and other interfering substances (you can look up a protocol on the internet).  I once know a person who was trying to do PCR from snails (which produce lots of mucous, and hence, mucopolysaccharides), and had trouble until he added CTAB to the extraction buffer.  If you are having trouble with PCR using  DNA from plants or organisms with lots of mucous, you could try adding CTAB to your extraction buffer.  Otherwise, I do not think most carbohydrates have much effect on PCR.

Glucose-6-phosphatase (G6Pase, Heparin  and undigested  proetoglycans hinder qunatification using real-time PCR (targeting 16S rDNA) .

Christopher is right, you need to purify your template, and CTAB is a good way to eliminate polysaccaride. I work with gelatinous animals (a lot of mucus), and I use CTAB phenol/chloroform extraction. The results are perfect!

Thanks Dr  Theodorakis.thanks for your full answer.

thanks dear Dr  Ravi Kant Upadhyay and Dr Aglieri.

Dear Maryam 

CTAB extraction method is used to hydrolysis the cell wall and removing the extra material as well as suger included in the structure of cell wall..Although some kits  based on extracton methods could be replaced to consume  time and energy.