Can anyone suggest how to design a transistor model in ansys circuit?

Can anyone suggest how to design a transistor model in ansys circuit. snp file with respect to different bias is available but from the datasheet I want to design transistor and observe it's...

05 March 2020 2,582 3 View

Which one is cheap diagnosis in FNAC and Ziehl-Neelsen Staing in the diagnosis of Tuberculous Lymphadenitis ?

FNAC is an assay which has high sensitivities when optimally selected is efficient and one of the most accurate frontline method for the diagnosis of Tuberculous Lymphadenitis. It is effectively...

23 January 2018 3,853 20 View

Can anyone provide me a suitable protocol for isolation of plant antimicrobial peptide?

Plants contains different types of peptides. Some of them are antimicrobial in nature which helps them fight against different microbes. If I want to isolate these peptides (Extracellular or...

08 November 2017 9,459 3 View

What should I do to pull a polar compound (Schiff base) up from the baseline on TLC?

I am trying to synthesize a schiff base by reacting aldehyde with amine, using DMSO as solvent (as it is only soluble in this solvent). Before doing TLC, the sample was diluted with ethyl acetate...

13 September 2017 8,372 3 View

How to avoid noise during image processing in Western Blot through chemiluminescence method?

We use ECL substrate and HRP enzyme.

08 August 2017 1,802 4 View

How long can GFP plasmid DNA withstand sonication without hampering its expression?

11 April 2017 2,179 3 View

How can i find out parity of a Bloch state at TRIM point?

Is there any way to find out parity from the band structure of First principle calculation ? Any kind of help will be appreciate. Thanks

04 December 2016 6,989 4 View

Which simulation software is more reliable for antenna design as well as mesh analysis among Ansoft HFSS, CST Microwave Studio and ADS Agilent?

Antenna design in one software varies with result in another software. Also which one is better for mesh analysis.

13 October 2016 9,190 12 View

Looking for Electromagnetics Books by Hollis C. Chen and Branislav Notoras

Hello, Can anybody please suggest where I can find the pdf of this book? TIA. Theory of Electromagnetic Waves: A Coordinate-free Approach Author: Hollis C. Chen MATLAB-Based...

01 January 1970 2,764 8 View

what is the simplest definition of ion exchange capacity

What this unit identifies for the ion exchange capacity ca. 0.92 meq. g-1. I am a newcomer in the membrane area. I just started. I highly appreciate your help

08 May 2021 0 0 View

what is the simplest definition of ion exchange capacity

What this unit identifies for the ion exchange capacity ca. 0.92 meq. g-1. I am a newcomer in the membrane area. I just started. I highly appreciate your help

08 May 2021 0 0 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

Dapi fluorescence protocol for colon cell lines ?

28 February 2021 2,534 2 View

Does RNAiMAX transfection reagent work in serum free medium?

I diluted siRNA and RNAiMAX in opti-MEM and added to the cells which they were in the growth medium. Is it a right way? or should I culture cells in the opti-MEM medium for a while and not in...

26 February 2021 10,041 3 View

Has anyone had luck using cell lines other than HEK 293 (such as COS1) to package lentivirus?

I am looking to package lentivirus in endothelial cells but I am opposed to using 293 cells due to personal ethics regarding the source. I have done some reading and saw that COS1, COS7, & CHO...

25 February 2021 3,030 1 View

Unknown debris sheets observed in cell culture flasks?

Hi, We culture neural progenitor cells in T175 culture flasks coated with PLO-Laminin. We have successfully grown these cells with our protocol for months. Recently, we observed large sheets...

25 February 2021 3,443 4 View

How do I transfect a large plasmid into PC12 cells with nerve growth factor (NGF) receptor?

I have a large 16 kb plasmid, which I need to transfect into PC12 cells. Lipofectamin 2000 didn't work and with GFP alone the transfection rate is very low. I also tried the neon invitrogen...

25 February 2021 6,635 5 View