there are many methods for that; it include: 1) culturing on CLED media, MacConkey agar, DCA agar 2) increasing concentration of agar 3) addition of 0.5-1.5 % of urea 4) using of alcohol treated plates 5) addition of some chemicals such as KAl(SO4)2
6) addition of some growth inhibitors such as sodium azide, boric acid, sulphonamides
Agar more concentrate, impedes its progress. And with deoxycholate (deoxycholate agar).
Agar concentration has to be increased to atleast 4 % or the good choice is grow in Macconkey agar...... You can surely prevent swarming
Thats also good idea of Shagufta Liaqat ....... If it is available it can be used... but all labs will surely have MacConkey agar.. Thats y i suggested
but in my experience, proteus swarms on MacConkey agar.... then usualy i need to use CLED medium...
oh ok when i worked with Proteus i usually use even nutrient agar or any basal medium with 4 % agar concentration....... However CLED is off course good choice.............
Routine Chrom Agar Medium (Urichrome) helps in identification and prevents swarming.
Here is another very simple method, that works well in fx blood agar or chocolate agar plates: Take the normal plate You intend to use, pour conc. ethanol (95 or 99%) over the plate - flood it. Let it stand say 0,5 min,, then drain the plate, and let the alcohol damp off several minutes, without cover. Your agarplate is then ready for seeding, susceptinility testing etc. without, -- or with very limited -- swarming :-)
@ Heltberg... ohh realy? dats great.... cozz proteus causes alot of problem on blood nd choclate agar.. dats a great idea, thanx :-)
Adding alcohol on the agar plate might do the trick but it will also dehydrate your agar at a faster rate than the ordinary. If you are using a mixed culture then definitely Proteus will be all over your plate. It would be best to isolate your organism of interest using a selective medium which will inhibit the growth of Proteus.
Reduce the amount of salt and increase the amount of agar. Mac plates work best if they have reduced salt content, which is why cled is also usefull
You can use Nutrient Agar check that it has not NaCl, or CLED agar that is specific for swarming inhibition.
Increase the concentration of agar, that is the best and easy method to prevent swarming
Bile salts are the conventional way of inhibiting swarming of Proteus. I just don't know whether this has not worked for you at all but I believe this from my long term experience it should work. Try Bile salt containing media
Indeed electrolyte deficient media agar inhibit swarming of Proteus; CLED is.and it manifests with clearly blue to blue green translucent colonies as an indication of its failure to ferment lactose so incorporated. However, the argument to increase agar concentrations in media should be handled a little more carefully as to put into consideration the ambient organism growth conditions for the best isolation and identification of the various strains.
there are many methods for that; it include: 1) culturing on CLED media, MacConkey agar, DCA agar 2) increasing concentration of agar 3) addition of 0.5-1.5 % of urea 4) using of alcohol treated plates 5) addition of some chemicals such as KAl(SO4)2
6) addition of some growth inhibitors such as sodium azide, boric acid, sulphonamides
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