When preserving and storing invertebrates in the laboratory for educational use (not scientific research), the key goals are to maintain specimen integrity, prevent decay, and ensure safety for handling by students. Here are commonly used methods depending on the type of invertebrate and duration of storage:

• Most invertebrates (insects, mollusks, annelids) are fixed initially in 70–90% ethanol or isopropanol. Ethanol is preferred as it preserves morphological features well and reduces mold growth.
• Avoid using formalin or formaldehyde for educational specimens unless absolutely necessary, due to toxicity and handling hazards.

• For short-term preservation, freezing specimens at −20°C can be used, but this may damage delicate structures and is less suitable for long-term display.

• For hard-bodied invertebrates like insects, drying via pinning or air drying is a classical method. This is excellent for teaching morphology but not for soft-bodied species.

• Use airtight, labeled containers filled with appropriate preservative (usually ethanol). Glass jars with tight lids work best to prevent evaporation.

• Specimens should have enough space in preservative fluid to avoid damage and ensure even preservation.

• Check preserved specimens periodically to top up preservative fluid, prevent evaporation, and look for mold or discoloration.

• Use non-toxic preservatives when possible and ensure proper labeling. Provide training for students on safe handling.

For educational collections, 70% ethanol storage is the most widely recommended and practical approach for long-term preservation of most invertebrates, balancing specimen quality, safety, and ease of use.

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