Protein-aptamer gel electrophoresis help, please??

Hi, I have problems with running gel electrophoresis. I have tried agarose gel electrophoresis and native PAGE. I have two proteins, which have molecular weights of ~30kDa and ~180kDa and two...

03 March 2021 4,275 4 View

Is there any tutorial for comsol general projections?

Hi, im trying to make a projection in a 3d comsol model. I have a non uniforme chanel and my intention is to get the average tempreture in the y-z cross section and se the average tempreture...

02 March 2021 4,624 4 View

Which is a lucid standard comprehensive book to guide on psychological scale construction and its standardization?

02 March 2021 7,082 3 View

What test should I use to compare two or three means, if data is not normally distributed but sample size is > 30 in each group?

I personally feel this as a most confusing question. As per my understanding, if sample size is > 30, and sample is skewed, we can still use paramteric approach, as it will follow Z...

01 March 2021 1,587 6 View

Expression cloning by using cDNA,do I need to modify the cDNA first?

I am going to have a expression cloning of mammalian gene by using shuttle plasmid to transforming the E.coli However I don't know I should only inserting the Coding sequence ,or I can...

28 February 2021 5,440 3 View

I need dataset for recommandation system?

Hi every body I need to collect datasets from different source to work on recommendation system algorithms, could you advise me how collect data?

27 February 2021 7,063 7 View

How to calculate Ligand-active site distance in a ligand-protein complex from MD simulation?

I have run a MD simulation of a ligand-protein complex in GROMACS using CHARMM forcefield. Reviewer has asked me to calculate Ligand-activesite distance and binding free energy of ligand with...

27 February 2021 4,703 1 View

Efficiency of Proximity Feed Patch Antenna ?

Greetings to all of You! I have searched some research papers for the designing of a proximity coupled patch antenna. Researchers are talking about the impedance bandwidth of the antenna but...

24 February 2021 7,662 3 View

How to estimate 2SLS with different instruments for different endogenous variables?

To be specific, I want to conduct an IV regression in stata with the following three equations. EQ1: Y1 ~Y2 Y3 X EQ2: Y2 ~ Z1 EQ3: Y3 ~ Z2 Both Y2 and Y3 are endogenous. However, Z1 and Z2 are...

23 February 2021 6,266 3 View

Which microtomy technique do you suggest for thin and delicate root sectioning ?And, how to preserve and process the roots before sectioning?

We have thin roots of from 1 year old different species of plants. We are looking into anatomical details of the roots. What's the best method to preserve and process the roots once they are...

23 February 2021 1,898 1 View

Glucose 6 phosphate dehydrogenase deficiency & NSAIDs - is this combination harmless ?

G6PD deficiency is quite common in our anesthesia practice in the middle east. Intraop/postop analgesia in them is an issue here. Many websites/books give contrasting advise with former advocating...

10 February 2021 4,817 3 View

Do you need permission to use a middle range theory in your dissertation?

I am using a figure of a middle range nursing theory, do I need permission to use?

03 February 2021 2,210 9 View

Any ideas on fast and easy way to stain mucus on intact cell layer?

Dear RG community, We are looking for a fast and easy way to stain mucus on a cell layer that allows for macroscopic visualization of mucus distrubution. Our epithelium is grown in conventional...

31 January 2021 6,790 2 View

Any one can tell me primer sequences used for q PCR to quantify MERS-CoV related coronavirus?

MERS-CoV primers

31 December 2020 2,389 2 View

Do you need participants for an online study in Hebrew?

Dear Israeli students and researchers, You are invited to post your studies on the subreddit for surveys/studies in Hebrew (for free): https://www.reddit.com/r/Surveys_in_Hebrew/ Hopefully this...

12 November 2020 292 3 View

How to collect data for Regional Research Study?

08 October 2020 8,003 4 View

Is it unethical to compare racial groups who are LGBT?

Hello everyone, I am currently conducting an undergraduate thesis on the unique challenges faced by Middle Eastern LGBT individuals due to their cultural background (often includes traditional...

08 October 2020 8,163 4 View

How to run a HSIAO test in STATA?

Hello friends, I am currently doing research about the determinants of financial self-sufficiency for moroccan MFIs, I have a data set of 6 individuals and 15 years. I learned that I have to run a...

01 October 2020 2,309 3 View

Automatic cell counting? (Ductal epithelium cells with fluorescence)?

Hello, I am currently conducting a lineage tracing experiment where I need to count GFP+ cells in multiple compartments of ductal epithelium (frozen tissue sections of an inducible Cre mouse with...

20 September 2020 1,855 6 View

What is the future of agricultural extension in the Middle East?

What is the future of agricultural extension in the Middle East?

10 June 2020 915 3 View