How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Binary Logistic Generalized Linear Mixed model in SPSS. Is it doable?

I want to analyses the proportion of swimming sperm of three species of fish in two salinities. To analyse the proportion of swimming sperm in a Generalized Linear Model, I would use a Binary...

03 March 2021 2,297 3 View

Looking for Materials with high thermal expansion coefficient and high temperature resistance?

03 March 2021 8,272 1 View

Guidelines for survey (question type) analysis?

Hi. Please tell me what guidelines should i need to follow for questionaries' type research work in India. It is not hospital based work, we are conduction basic institutional based qualitative...

03 March 2021 2,037 3 View

What are the values needed for gabor filter's parameter for cifar10 data?

Hi, I implemented a code to gabor filter cifar10 data but the images after being filtered and stacked are not clear like the original images. I think the problem is in the way I am using the...

03 March 2021 6,317 1 View

Can we block unwanted images during the x-ray prediction?

i am try to classify the x-ray images. During classification , can i block unwanted images (except x-ray image).

03 March 2021 7,100 1 View

No title

03 March 2021 5,360 2 View

What techniques can I use to determine the miscibility of polymer blends?

The term miscibility refers to the single-phase state in thermodynamics. I do not mean the compatibility of different components. To determine the miscibility I know several techniques such as...

03 March 2021 4,107 4 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

The procedure compute?

02 March 2021 5,204 3 View

Advice on growing lactobacillus species in the lab?

Hello everyone, For part of my PhD project, I'm hoping to grow some lactobacillus species in the lab so that I can create standard curves for a qPCR quantification of a faecal sample. However, I...

24 February 2021 3,157 6 View

How do you quantify bacterial qPCR data?

Hi all, How would you use qPCR to quantify the amount of a particular bacterial species in a sample experimentally? I understand you could use specific primers for the 16s V region of a...

01 February 2021 7,153 4 View

What is the best pH to grow Lactobacillus rhamnosus?

Hi all, I'm doing some research in the proteins secreted by L rhamnosus and I've been trying to optimise the fermentation conditions to maximise production of these proteins. Without pH control...

08 October 2020 4,647 6 View

Why Lactobacillus acidophillus is sensitive to Vancomycin but L. rhamnosus and casei are intrinsically resistant?

Does it have, anything to do with their fermentation status? I know that in several species of LAB, the terminal d-alanine residue is replaced by d-lactate or d-serine in the muramylpentapeptide,...

19 September 2020 3,242 2 View

Acceptable CV% and SD for results between stool samples in qPCR?

What would be the acceptable CV% and SD for copy number results between stool samples in qPCR (same group)? I am evaluating different bacteria (E.coli, Lactobacillus, etc.) using the absolute...

06 August 2020 8,112 4 View

I am looking for this strain, Lactobacillus acidophilus (strain for minicells production). Please can you help me to find it?

I am a master student, I need this strain for research purposes. Lactobacillus acidophilus [ minicells producing strain (Minicells are generally produced by aberrant cell divisions at...

13 March 2020 3,204 3 View

Why do Lactobacillus spp. not pellet in 4% PFA?

I am currently staining and fixing a range of bacteria for a glycobiologist. I am fixing the bacteria in 4% PFA and then washing with PBS. After fixing Lactobacillus spp. in PFA, it wont pellet. I...

02 February 2020 747 1 View

Why do I get higher copy number from qPCR with phylum specific primer than universal 16s primers?

Hi, My team was trying to profile bacterial phylum composition in food sample using qPCR. We used 16s universal primers and primers specific for certain phylum such as Firmicutes and...

01 February 2020 8,482 3 View

How Red colonies came after Sub culturing ?

Research enigma! Cloning in lactobacillus! After transformation I got complete white colonies but after sub culturing the same , It is starting to see red colonies.No idea from where I got those...

20 January 2020 6,401 3 View

Can someone help me with a plasmid miniprep protocol for L. lactis (Gram positive)?

Hello fellow researchers. I am struggling to find a good plasmid miniprep protocol for Gram-positive bacteria, specifically Lactococcus lactis. I have followed several protocols on PubMed, and I...

31 December 2019 2,036 1 View