Dear Hawraa Khalid,

from the text provided I understand, that you wish to observe your specimen (acryl?) under scanning electron microscope (SEM) after the transverse strenght measurement experiment.

Conducting the SEM observations strongly depends on the scientific question that you wish to answer employing such method. It depends on your sample (acryl? should be fine with additional coating) and also on the microscope available. Most probably you will start with observation of the fractured surface, to possibly identify any weak points, where the crack propagation started. The steps before actual SEM experiment will (most probably) include sample formatting, fixation on the substrate, coating with conductive layer. As you are (most probably) not interested in chemical composition of your sample, at this point you can focus yourself on SEI (secondary electrons), which will give you an information on the surface topography of your sample.

I suggest starting with some overview literature or online sources for getting an idea on the possibilities of the SEM on resin composites (like Polymer Microscopy by Sawyer L, Grubb D &, Meyers G F).

Some effort in formulating your problem will be appreciated. So far it's mostly meaningless.