I would like to be able to grow Candida without purchasing and will like to know the best sources for it and if not Candida then any other yeast cells. I am trying to test the antifungal properties of certain plant extracts on these yeast cells.
You can easily isolate Candida albicans, and Malassezia from clinical samples, and Rhodotorula and Cryptococcus neoformans from environmental materials (avian dropping) on Pal sunflower seed medium.We had developed Pal sunflower seed medium in 1980 for raid isolation and presumptive identification of Cr.neoformans from clinical and environmental samples.We have over 52 papers on Cryptococcus neoformans and several papers on Candida albicans and few papers on Malassezia.You can use our Narayan stain to study the detailed morphology of fungal isolates. You may please see the details of Pal sunflower seed medium from our many publications uploaded on Research Gate.In case you find any problem,you may contact me on my email: [email protected]
I wish you best of luck in your academic career.
Prof.Dr.Mahendra Pal
Founder Director of Narayan Consultancy on Veterinary Public Health and Microbiology, Anand, India