What are the best quenchers for eGFP?

10 October 2013 1 10K Report

I'm planning an assay based on the quenching / FRET with eGFP as partner. Could somebody point me to a few efficient Quenchers (or a review regarding that matter?).

Leon D Islas

DPA (Dipicryl amine or hexanitrodiphenylamine) is a very efficient quencher of GFP/YFP. It is a hydrophobic anion that intercalates in the plasma membrane, so, if your constructs are membrane-associated, this is a very good system.

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