Need 0.5-1000ng/ml sensitivity. Any experience? Brands to use?
See the following papers by our Univ Colleagues
https://www.jstage.jst.go.jp/article/jset/9/2/9_2_141/_article/-char/ja/
and
Kinoshita, M., Zakaria, M. P., Ismail, A., Yusof, S., Boonphakdee, C., Boonphakdee, T., & Inoue, K. (2010). An attempt to detect contamination with estrogenic compounds in river water of urban area in Thailand and Malaysia using transgenic medaka. J. Coast. Mar. ScI, 34(1), 216-222.
You can go throw this article which will be help full for you.
Rapid Detection of Selected Steroid Hormones from Sewage Effluents using an ELISA in the Kuils River Water Catchment Area, South Africa https://doi.org/10.1080/15321810701603799
ELISA will probably work, currently the most commonly used test system for estrogens is ER CALUX® from Biodetection systems. You can also get a variant for androgenic compounds etc. etc. I think this test system can do the concentration range You ask without preconcentration. real concentrations in wastewater are often around 1 ng/L, though. - Then You would need a preconcentration.
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