I want to know best biomarkers useful for satellite muscle cells.
How can I verify my cells are satellite muscle cells?
Dear Ashkan,
Classically, satellite cells were identified based on a unique anatomical location: beneath the surrounding basal lamina and outside the myofiber plasma membrane. This anatomical location gives satellite cells a “wedged” appearance when viewd by electrictron microscropy. This technique also revealed other morphological characteristics of satellite cells: large nuclear-to-cytoplasmic ratio, few organelles, small nucleus, and condensed interphase chromatin. This morphology is in harmony with the notion that most satellite cells, in healthy unstressed muscles, are mitotically quiescent (in G0 phase) and transcriptionally inactive. In addition to electron microscopy, satellite cells can be identified by phase-contrast microscopy on single myofiber explants. Based on the same principle, the behaviors of satellite cells on single myofiber explants can be recorded by utilizing live cell imaging techniques. The identification of satellite cells by fluorescence microscopy relies on specific biomarkers
Among biomarkers are: .α7-intergin, CD34 and etc. For more biomarkers see Figure 1 in the publication described in the following link:
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4073943/
Hoping this will be helpful,
Rafik
measuring the expression of PAX7 could help you to identify satellite cells
Satellite Cells and Skeletal Muscle Regeneration
ARTICLE in COMPREHENSIVE PHYSIOLOGY 5(3):1027-1059 · JULY 2015 with 505 READS
Impact Factor: 4.74 · DOI: 10.1002/cphy.c140068 · Source: PubMed
Pax7 and alpha7 integrin. Do you want to identify them in culture or in vivo? Anyway, while quiescent or proliferating they express Pax7.
- Badges
- Science topic
- Similar topics
- Biological Science
- Molecular Cell Biology