One way to enumerate the number of bacteria present in soil sample is to utilize dilution and plating...........Once the bacterial colonies have grown on the agar plates, isolate the pure cultures of selected colonies by using streak plates technique. Such pure cultures can be further analyzed and characterized for specific traits and functions. To isolate both fungi and bacteria you may use Thortons media or Potato Dextrose Agar ( PDA ) media. Nutrient agar can be employed. .... For more details consult https://www.jove.com -- science - education