Buffer for protein solubilization after acetone/AS precipitation that is compatible with cell stimulation?

Hello! I am precipitating some proteins (with either acetone or ammonium sulphate) and I need to resuspend the protein pellet in a buffer that properly keeps the proteins soluble AND which is...

27 January 2021 6,958 4 View

How to genotype the arabidopsis mutant eds5-1? How to find the exact place of the SNP?

How to genotype eds 5-1 arabidopsis mutant? It's SNP but no idea where it is. Articles were found only with contradictory primer sets. Every articles are citing the same author and journal...

25 June 2018 4,559 3 View

Best way for His-tag purification?

Hello, I would go for His-tag purification and I was wondering which company would be the best to order Ni-TA column? Do you have a preferred one or all of them would be the same? Thanks in...

03 April 2017 6,420 4 View

Sf9 cells won't transfect... Any suggestions on how to improve?

I am working with the bac-to-bac expression system and following the Invitrogen protocol available online. I have generated my recombinant bacmid and now I should transfect my cells, though they...

16 May 2016 4,906 2 View

Are NK92MI cells smaller when they are in low cell number?

I am trying to culture NK92MI cells, but they seem to be extremely small. I tried to subculture them in T25 flask (as described by ATCC ~200 000 cell/ml, in the complete growth medium they...

12 March 2015 2,218 2 View

Does anyone have an idea what can be the problem during our RNA ISH on free-floating mouse brain sections?

22 February 2015 4,855 7 View

What is the most important thing in your life?

maybe science, family, learning?

01 January 1970 3,812 82 View

Is it better to attend a conference or just participate?

I also like to perform.

01 January 1970 3,416 19 View

How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Guidelines for survey (question type) analysis?

Hi. Please tell me what guidelines should i need to follow for questionaries' type research work in India. It is not hospital based work, we are conduction basic institutional based qualitative...

03 March 2021 2,037 3 View

How to access MSCI KLD Stats and Sustainalytics?

Hello all, I'm currently working on my undergraduate thesis and I'm having difficulties trying to access KLD Stats and Sustainalytics because my campus Airlangga University and apparently other...

02 March 2021 8,525 1 View

Ideas for Phd's Thesis on pricing (Marketing)?

Hi everyone, I'm studying Marketing and I would like to write my PhD thesis on the topic of pricing. Any specific ideas?

02 March 2021 9,706 5 View

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02 March 2021 3,060 3 View

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02 March 2021 932 1 View

How to calculate BREQ 3 questionnaire?

how to calculate BEHAVIOURAL REGULATION IN EXERCISE QUESTIONNAIRE BREQ 3 ,which contain 24 questions and each question has 5 answers (0- Strongly disagree , 1-disagree, 2- Neither agree nor...

02 March 2021 811 3 View

Please suggest a good text book for "Research Methodology in Life Sciences". Which book would be good for PhD Scholars?

Please provide Book Title and author name

02 March 2021 9,059 3 View

How does Scientist/RG community evaluates a case study article and the impact of it on a researcher career?

An article that is published as a case study in the SJR listed journal. How much value is added to a researcher career? How it is evaluated by a scientific researcher/University admission...

02 March 2021 9,661 3 View

Could any one guide me, to write research proposal for grant? ?

We have one plant, the local people using this for controlling blood sugar, the toxicity and antidiabetic activity of the plant is not reported yet, please guide from whag should be the process ?...

02 March 2021 2,300 1 View