I think these are bacteria. But depending on colony characteristics it's quite difficult to guess the bacterial genus.

Sulav Indra Paul Thanks for answering. Yes, I'm also thinking it could be a bacteria. You're right, its not easy to identify its genus unless if i run some test like Gram staining.

I also think that these are bacteria. But it is so hard to guess the bacterial genus by observing colony morphology. For determining bacterial genus you can do some test, you can follow Bergey's Manual of Systematic Bacteriology.

It is easy to tell when you see them under the microscope.

Md. Niuz Morshed Khan Yes, I'm thinking the same too. I will run some biochemical test to identify the bacteria.

Thank you for the suggestion.

I will look into it.

Francisco Javier Alvarez

Thank you for the suggestion Sir.

After the Gram Staining , I will have to observe slide under the microscope.

Dear Banupriya, if you really want to know the species of bacteria or fungi in your plates, the best way would be to run a PCR with primers for the 16S ribosomal gene and send the PCR product for sequencing. Good luck with everything.

Hi Banupriya

Yes, bacteria,

To know the exact type of bacteria you have variety of things to do e.g some chemical tests, or make a slide from your sample with gram stain ,and of course some colonies have specific traits such as proteus spp which have fishy odor , the last but not the least is by doing PCR.

I recommend the microscope examination. You can easily find if colonies are formed by bacteria or if they are fungal ones.

After this, you can start the identification of bacteria, if the colonies are bacterial.

The bacterial identification can be performed in many ways. Everything depends on the equipment in your laboratory and your capabilities.

Best regards

Vit