How to check spread of AAV2 virus that doesn't express fluorophores?

Hi, I am injecting AAV2-Retro virus (AAV2-retro-EF1a-DIO-FLPo-WPRE-hGHpA from Addgene) that carries cre-dependent flp recombinase, in the mouse dorsal hippocampus and I need to verify that the...

08 November 2020 9,559 3 View

May I know, which statistical analysis and conceptual framework will be suitable for my study?

Helo, I am interested in looking into Secondhand smoke exposure and support level towards smoke-free legislation. I am planning to use secondary data. Can anyone advice on which statistical...

23 May 2020 6,381 2 View

How to evaluate the In vitro study for coronavirus (COVID 19 Inhibitory activity)?

Dear Researchers .... Need help for COVID 19 drug discovery. Recently, COVID-19 main protease crystal structure was deposited in the protein database (PDB ID: 6LU7...

08 March 2020 3,853 4 View

How to upload homology modeled 3D protein in online free protein bank website?

Dear research professors and scholars, I have developed a novel 3D protein structure (mutant DNA Gyrase enzyme of antibiotic-resistant E. coli) by homology modeling technique. Because this type of...

21 September 2019 5,159 2 View

409bp is good for real time PCR?

I designed a primer and probe for real time PCR using (https://www.eurofinsgenomics.eu/en/ecom/tools/qpcr-assay-design/) this website with the bp size shows 143. after i received the primer i did...

19 July 2019 7,963 10 View

How to reduce NO2 to NH2 without affect any other group in following reaction?

Hi, I have to reduce the NO2 group to NH2 group in the following reaction. Kindly suggest some good catalyst to reduce the NO2 to NH2 group without affecting any other position.

20 December 2017 8,046 51 View

How to run molecular dynamics simulation for protein in discovery studio 3.5 software?

Hi, I have planned to study of molecular dynamics simulation for my homology modeled protein. Generally, most of Molecular dynamics (MD) simulations were carried out using GROMACS software. But i...

27 November 2017 3,571 5 View

May I get journals regarding Ectropothecium sp, Dicranoloma sp and Mitthyridium constrictum mosses please?

My friend's final year project is finding bioactive potential of liverworts and mosses. She is lacking of reference to complete her literature review. Can anyone help us to get access for the...

17 April 2017 2,416 6 View

How the surface oxide layer involved in the corrosion resistance?

How the surface oxide layer involved in the corrosion resistance?

19 June 2016 5,808 10 View

Could any one suggest studies that looked into the function of rat/mouse hippocampal commissures?

Hello, I have a hard time finding studies that looked into the functions of hippocampal commissures in rats or mice. Any suggestions on relevant studies would be appreciated. Thanks Mani

31 May 2016 280 3 View

Which is the best place in the world for the plant biotechnology research?

I am searching for a good place for the Post Doc,

02 March 2021 4,053 3 View

Which bacteria are suitable for quorum sensing screening?

I want to find a quorum sensing inhibitor from plant extracts. Klebsiella pneumoniae, Moraxella catarrhalis, Staphylococcus aureus, and Streptococcus pneumoniae. I looked for these strains...

01 March 2021 7,675 3 View

Isolates of Hymenoscyphus fraxineus?

Dear Dr. Cai, my name is Simone Prospero and I work in the team of Phytopathology at the Swiss Federal Institute for Forest Snow and Landscape Research (WSL;...

01 March 2021 1,133 1 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

Where to find data on trees in tropical agroforestry?

Hi, I'm looking for data (mainly related to management: growth rate, canopy size, soil and climate preferences, etc.) about tropical trees used in tropical agroforestry. Have you ever heard about...

28 February 2021 7,356 8 View

How to remove fatty material from plant extract?

The resultant solid was partitioned three times with 250 ml of petroleum ether (40-60/c) to remove any fatty material and evaporated to dryness.

28 February 2021 9,464 2 View

Good morning research fellows, Has anyone worked with senna alata plant extract on any kind of fish?

Literatures on the piscicidal effect of sennaa alata is scanty. if you have any related litrature either in the bark, leaves or roots used as treatment to any aquatic organism please kindly help...

28 February 2021 9,138 2 View

What HPLC Marker(s) should be used to detect anthocyanins in a sample?

What HPLC Marker(s) or standard is used to detect and quantify anthocyanins such as Delphinidin, Cyanidin in a given sample of plant extract?

23 February 2021 1,832 4 View

Can two EC50 values with different hill slopes be compared?

Hello, I fit a classical dose response curve Y=Bottom + (Top-Bottom)/(1+10^((LogEC-X)*HillSlope)) to my data (not related to inhibitors etc). The model I use is purely qualitative but it fits...

23 February 2021 8,155 5 View

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22 February 2021 9,932 11 View